Clock (E270) Polyclonal Antibody-BS1873

Clock (E270) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS1873-50, BS1873-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O15516

Host: Rabbit

Reactivity: Human

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: Clock, a member of the basic-helix-loop-helix-PAS (bHLH-PAS) family of transcription factors, has also been identified as having circadian function. Mutations within the Clock gene have been shown to increase the length of the endogenous period and to cause a loss of rhythmicity of circadian oscillations. Clock contains a DNA-binding domain, a protein dimerization domain and a glutamine-rich C-terminal region, which indicates transactivation capabilities. It has been speculated that Clock may regulate circadian rhythmicity in combination with other proteins such as Per.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Clock (E270) polyclonal antibody detects endogenous levels of Clock protein.

Bioworld Molecular Weight: ~ 100 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Clock (E270) pAb at 1:500 dilution
Lane 1: A549 whole cell lysate (40ug)
Lane 2: HEK293T whole cell lysate (40ug)
Lane 3: A2780 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of Clock (E270) pAb in paraffin-embedded human colon carcinoma tissue at 1:5000. Cytoplasm and Nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of Clock (E270) pAb in paraffin-embedded human colon carcinoma tissue at 1:5000. Cytoplasm and Nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Alternative Name: Circadian locomoter output cycles protein kaput; hCLOCK; Class E basic helix-loop-helix protein 8; bHLHe8; CLOCK; BHLHE8; KIAA0334

Immunogen: Synthetic peptide, corresponding to amino acids 241-290 of Human Clock.

Conjugate: Unconjugated

Modification: Unmodified