CNN1 Polyclonal Antibody
Catalogue Numbers: BS60580-50, BS60580-100
Sizes: 50µl, 100µl
Alternative Name: Calponin-1; Basic calponin; Calponin H1, smooth muscle; CNN1
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P51911
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: Calponin regulates smooth muscle cell contraction and is a marker of smooth muscle cell differentiation. Calponin, an Actin- and Tropomyosin-binding protein, is characterized as an inhibitory factor of smooth-muscle actomyosin activity. Calponin is implicated in the regulation of smooth muscle contraction through its interaction with F-Actin and inhibition of the Actin-activated MgATPase activity of phosphorylated Myosin. Both properties are lost following phosphorylation (primarily at Serine 175) by protein kinase C or calmodulin-dependent protein kinase II. The three forms of Calponin, Calponin 1 (basic Calponin), Calponin 2 (neutral Calponin) and Calponin 3 (acidic Calponin), are found in smooth muscle tissue. Additionally, Calponin 2 is found in heart muscle tissue and Calponin 3 is found in the brain.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: CNN1 polyclonal antibody detects endogenous levels of CNN1 protein.
Bioworld Molecular Weight: ~ 33 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of CNN1 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: H9C2 whole cell lysate
Immunogen: A synthetic peptide corresponding to residues in Human CNN1
Conjugate: Unconjugated
Modification: Unmodified