Product Description

CNN1 Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9892M-50, BS9892M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P51911

Host: Rabbit

Reactivity: Human, Mouse, Rat, Dog, Pig

Applications: WB, ICC/IF, IHC

All Applications: WB: 1:1000-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200

Background: Calponin regulates smooth muscle cell contraction and is a marker of smooth muscle cell differentiation. Calponin, an Actin- and Tropomyosin-binding protein, is characterized as an inhibitory factor of smooth-muscle actomyosin activity. Calponin is implicated in the regulation of smooth muscle contraction through its interaction with F-Actin and inhibition of the Actin-activated MgATPase activity of phosphorylated Myosin. Both properties are lost following phosphorylation (primarily at Serine 175) by protein kinase C or calmodulin-dependent protein kinase II. The three forms of Calponin, Calponin 1 (basic Calponin), Calponin 2 (neutral Calponin) and Calponin 3 (acidic Calponin), are found in smooth muscle tissue. Additionally, Calponin 2 is found in heart muscle tissue and Calponin 3 is found in the brain.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of CNN1 and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 33 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CNN1 Rabbit mAb at 1:1000 dilution
Lane 1: Hela whole cell lysate, Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Calponin antibody. Counter stained with hematoxylin., ICC staining Calponin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.

Alternative Name: Calponin-1; Basic calponin; Calponin H1, smooth muscle; CNN1; Calponin1; Calponin-1;

Immunogen: Recombinant antibody.

Conjugate: Unconjugated

Modification: Unmodified