Product Description

CNPY2 Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS71704-50, BS71704-100

Swiss-Prot: Q9Y2B0

Host: Rabbit

CNPY2 Polyclonal Antibody-BS71704

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200

Background: Positive regulator of neurite outgrowth by stabilizing myosin regulatory light chain (MRLC. It prevents MIR-mediated MRLC ubiquitination and its subsequent proteasomal degradation.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 21kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using CNPY2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s., Immunohistochemistry of paraffin-embedded mouse kidney using CNPY2 Rabbit pAb at dilution of 1:100.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Alternative Name: CNPY2; HP10390; MSAP; TMEM4; ZSIG9

Immunogen: Recombinant fusion protein of human CNPY2(NP_055070.1).

Modification: Unmodified

Conjugate: Unconjugated