Cochlin Polyclonal Antibody-BS67728

Cochlin Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS67728-50, BS67728-100

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: O43405

Host: Rabbit

Reactivity: Human

Applications: WB, IHC, IF/ICC

All Applications: WB (1/500 - 1/1000), IHC (1/50 - 1/100), IF/ICC (1/50 - 1/100)

Background: Plays a role in the control of cell shape and motility in the trabecular meshwork.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of Cochlin protein.

Bioworld Molecular Weight: ~ 65 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Cochlin expression in HEK293T (A) whole cell lysates., Immunohistochemical analysis of Cochlin staining in human prostate formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunofluorescent analysis of Cochlin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Alternative Name: COCH5B2; Cochlin; COCH-5B2

Immunogen: Recombinant fusion protein of human Cochlin. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified