Product Description

Cofilin 1 (phospho-S3) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS5063-50, BS5063-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Cofilin is ubiquitously expressed in eukaryotic cells where it binds to Actin, thereby regμlating the rapid cycling of Actin assembly and disassembly, essential for cellμlar viability. Cofilin is a low molecμlar weight protein that binds to filamentous F-Actin by bridging two longitudinally-associated Actin subunits, changing the F-Actin filament twist. This process is allowed by the dephosphorylation of Cofilin Ser 3 by factors like opsonized zymosan. LIM kinase 1 (LIMK-1), a serine kinase, phosphorylates Cofilin and renders it unable to bind and depolymerize F-Actin.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: IHC

Alternate Names: Cofilin-1, 18 kDa phosphoprotein, p18, Cofilin, non-muscle isoform, CFL1, CFL

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human Cofilin 1 around the phosphorylation site of Serine 3.

Dilution: IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-COFILN1 (S3) polyclonal antibody detects endogenous levels of COFILN1 protein only when phosphorylated at Ser3.

Molecular Weight: ~ 19 kDa

Pathway: Phosphoinosotode Signaling, Regμlation of Actin Dynamics, Tumor Angiogenesis, Regμlation of Microtubμle Dynamics, B cell receptor signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P23528

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only