Cofilin Monoclonal Antibody-MB11974

Cofilin Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB11974-50, MB11974-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: P23528

Host: Rabbit

Reactivity: Human

Applications: WB, IHC-F, IHC-P, ICC/IF

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200

Background: The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 19 kDa; Observed MW: 19 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Cofilin in A549, MCF-7, 293T lysates using Cofilin antibody., Immunocytochemistry analysis of Cofilin in hela using Cofilin antibody, and DAPI, Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Cofilin antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: CFL1; CFL; Cofilin-1; 18 kDa phosphoprotein; p18; Cofilin; non-muscle isoform

Immunogen: A synthetic peptide of human Cofilin

Conjugate: Unconjugated

Modification: Unmodified