Product Description

Collagen 10 alpha 1 Polyclonal Antibody

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Catalogue Numbers: BS67344-50, BS67344-100

Sizes: 50µl, 100µl

Swiss-Prot: Q03692

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Type X collagen is a product of hypertrophic chondrocytes and has been localized to presumptive mineralization zones of hyaline cartilage.

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Recognizes endogenous levels of Collagen 10 alpha 1 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Collagen 10 alpha 1 expression in MCF7 (A) whole cell lysates., Immunohistochemical analysis of Collagen 10 alpha 1 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~ 70 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Collagen alpha-1(X) chain

Immunogen: Recombinant full length protein of human Collagen 10 alpha 1

Conjugate: Unconjugated

Modification: Unmodified