Product Description

Collagen 5 alpha 1 Polyclonal Antibody

Catalogue Numbers: BS67487-50, BS67487-100

Sizes: 50µl, 100µl

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: P20908

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB (1/500 - 1/1000)

Background: The Extracellular Matrix (ECM) is a complex network of macromolecules that provides structural tissue support to cells in the basement membrane and interstitial matrix. It is composed of many molecules including proteins, glycoproteins, proteoglycans, and polysaccharides . One of the major proteins that comprises the ECM, and the human body, is collagen. Collagens are a large family of proteins. They are trimeric molecules comprised of three alpha polypeptide chains that form a triple helix structure that is characteristic of all collagens . The large family of collagens is divided into three subgroups: the fibrillar collagens, the non-fibril forming collagens, and the fibril-associated collagens. These sub groups differ in their structure and supramolecular assembly . COL5A1 is a minor fibrillar collagen expressed broadly across tissues. It participates in bone and cartilage development, and defects in the COL5A1 gene leads to Ehlers-Danlos Syndrome, a condition that affects the integrity of bones, cartilage, and skin . Several proteomics, gene profiling, and biochemical studies have indicated that aberrant COL5A1 may play a malignant role in various types of cancer including breast, renal, colon, prostate, lung, and others .

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of Collagen 5 alpha 1 protein.

Extra Notes: Western blot analysis of Collagen 5 alpha 1 expression in C6 (A), CT26 (B) whole cell lysates.

Bioworld Molecular Weight: ~ 180 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Collagen alpha-1(V) chain

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Collagen 5 alpha 1. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified