Product Description

Collagen I/COL1A1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79834-50, BS79834-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P02452

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC, IHC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200 | IHC,1:50 - 1:200

Background: This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 140KDa/220KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Rat skin, using CollagenI/COL1A1 antibody at 1:850 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s., Western blot analysis of U-118 MG, using CollagenI/COL1A1 antibody at 1:850 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s., Immunohistochemistry of paraffin-embedded human lung cancer using Collagen I/COL1A1 Rabbit pAb at dilution of 1:150. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded mouse kidney using Collagen I/COL1A1 Rabbit pAb at dilution of 1:150. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Alternative Name: COL1A1; EDSC; OI1; OI2; OI3; OI4

Immunogen: A synthetic peptide of human Collagen I/COL1A1(NP_000079.2).

Conjugate: Unconjugated

Modification: Unmodified