Product Description

Collagen VI Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90318-50, BS90318-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P12109(Human) P12110(Human) P12111(Human) Q02788(Mouse) Q04857(Mouse) Unigene:232118(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC

All Applications: WB:1:1,000-1:2,000
ICC:1:100-1:500
IHC:1:50-1:200

Background: This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Collagen VI polyclonal antibody detects endogenous levels of Collagen VI protein.

Bioworld Molecular Weight: 134/109 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Collagen VI on different lysates using anti-Collagen VI antibody at 1/1,000 dilution.
Positive control:
Lane 1: Mouse heart
Lane 2: Human heart
Lane 3: Human kidney, ICC staining Collagen VI in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: COL6A1, COL6A2, COL6A3, Alpha 1 (VI) chain (61 AA), CO6A1, COL6A1, COL6A2, COL6A3, Collagen alpha 2(VI) chain, Collagen alpha 3(VI) chain, Collagen alpha-1(VI) chain, Collagen type VI alpha 1, Collagen type VI alpha 2, Collagen type VI alpha 3, Collagen VI alpha 1 polypeptide, Collagen VI alpha 2 polypeptide, Collagen VI alpha 3 polypeptide, CollagenVI, Human mRNA for collagen VI alpha 2 C terminal globular domain, OPLL, PP3610,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified