COMMD5 Polyclonal Antibody-BS72895

COMMD5 Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS72895-50, BS72895-100

Swiss-Prot: Q9GZQ3

Host: Rabbit

COMMD5 Polyclonal Antibody-BS72895

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: May modulate activity of cullin-RING E3 ubiquitin ligase (CRL complexes. Negatively regulates cell proliferation. Negatively regulates cell cycle G2/M phase transition probably by transactivating p21/CDKN1A through the p53/TP53-independent signaling pathway. Involved in kidney proximal tubule morphogenesis (By similarity. Down-regulates activation of NF-kappa-B.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 25kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using COMMD5 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s., Immunofluorescence analysis of C6 cells using COMMD5 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: COMMD5; HCARG; HT002

Immunogen: Recombinant fusion protein of human COMMD5(NP_054785.2).

Modification: Unmodified

Conjugate: Unconjugated