COMT Polyclonal Antibody-BS5671

COMT Polyclonal Antibody

Catalogue Numbers: BS5671-50, BS5671-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P21964

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Catechol-O-methyltransferase (COMT) plays a crucial role in the regulation of central dopaminergic systems by catalyzing the inactivation of catechol-amines. It is widely distributed in most tissues in soluble and membrane-bound forms. COMT-mediated methylation metabolism of catecholamine neurotransmitters is a first-line detoxification pathway. A Val158Met polymorphism of the COMT gene affects activity of the enzyme and influences performance and efficiency of the prefrontal cortex of the brain. Sequential conversion of estradiol to methoxyestradiol by COMT, contributes to the antimitogenic effects of estradiol on vascular smooth muscle cell growth via estrogen receptor-independent mechanisms. COMTD1 (catechol O-methyltransferase domain-containing protein 1) is a 262 amino acid single-pass type II membrane protein that belongs to the methyltransferase superfamily.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: COMT polyclonal antibody detects endogenous levels of COMT protein.

Extra Notes: Western blot (WB) analysis of IL3RA pAb at 1:500 dilution
Lane1:Myla2059 whole cell lysate(20ug)
Lane2:U-87MG whole cell lysate(40ug)
Lane3:HepG2 whole cell lysate(40ug)
Lane4:HUT78 whole cell lysate(40ug)
Lane5:L02 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 30 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Catechol O-methyltransferase

Immunogen: Synthetic peptide, corresponding to amino acids 111-156 of Human COMT.

Conjugate: Unconjugated

Modification: Unmodified