Product Description

Connexin 43 (phospho-S367) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4777-50, BS4777-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Connexin 43 (Cx43) is a member of the large family of gap junction proteins. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regμlation of cell growth. Phosphorylation of Cx43 is important in regμlating assembly and function of gap junctions. Ser367 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication. Src can interact with and phosphorylate Cx43 to alter gap junction communication.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: Gap junction alpha-1 protein, Connexin-43, Connexin 43, Connexin43, Cx43, Cx-43, Gap junction 43 kDa heart protein, GJA1, GJAL

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human Connexin 43 around the phosphorylation site of Serine 367.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-Connexin 43 (S367) polyclonal antibody detects endogenous levels of Connexin 43 protein when phosphorylated at Ser367.

Molecular Weight: ~ 43 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P17302

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only