Product Description

Connexin 43 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90322-50, BS90322-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P17302(Human) P23242(Mouse)

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC FC

All Applications: WB:1:500-1:2,000
IHC:1:50-1:200
FC:1:50-1:200

Background: The connexins are a group of gap junction proteins which form a hexamer to compose a connexon. Clusters of connexons form a gap junction through which low molecular weight proteins may diffuse from cell to cell. Several mammalian cells with malignant phenotypes exhibit decreased connexin expression and gap junction communication. In Src transformed cells, there is a decrease in gap junctional communication, which appears to be associated with tyrosine phosphorylation of connexin 43. Activated c-Src phosphorylates the C-terminal tail of connexin 43 on Tyr 265, resulting in a stable interaction between both proteins, which leads to inhibition of gap junctional communication. In addition to tyrosine phosphorylation, connexin 43 has also been shown to be phosphorylated on serine in the absence of Src kinases and on both serine and tyrosine in cells expressing Src kinases, such as c-Src and/or pp60v-Src. In human vascular endothelial cells, connexin 43 is posttranslationally modified during mitosis. Mitosis-specific phosphorylation of connexin 43 correlates with the transient loss of gap junction intercellular communication and redistribution of connexin 43.

Purification and Purity: Peptide affinity purified.

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: Connexin 43 polyclonal antibody detects endogenous levels of Connexin 43 protein.

Bioworld Molecular Weight: 43 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Connexin 43 on mouse testis tissue lysate using anti-Connexin 43 antibody at 1/2,000 dilution., Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Connexin 43 antibody. Counter stained with hematoxylin.

Alternative Name: Cx43, GJA1, GJAL, Connexin 43, Connexin-43, Cx 43, Cx43, CXA1, DFNB38, Gap junction 43 kDa heart protein, Gap junction alpha-1 protein, Gap junction protein alpha 1 43kDa (connexin 43), Gap junction protein alpha 1 43kDa, Gap junction protein alpha like, GJA 1, Gja1, GJAL, ODD, ODDD, ODOD, SDTY3,

Immunogen: Synthetic peptide within C-terminal human Connexin 43.

Conjugate: Unconjugated

Modification: Unmodified