Product Description

Cot (P284) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1074-50, BS1074-100

Sizes: 50µl, 100µl

Swiss-Prot: P41279

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: The role of mitogen-activated protein kinases (MAPKs) in cell signaling pathways is well established. The rat gene Tpl-2, for tumor progression locus 2, and the human and mouse homologues c-Cot, for cancer osaka thyroid oncogene, encode a proto-oncogene serine/threonine protein kinase that was shown to play a role in the functional activation of the MAP kinase pathway. Overexpression of Cot induces MAP kinase activation in COS-1 and NIH/3T3 cells. Cot-mediated activation of MAP kinase is inhibited by both Ras N17, a dominant negative mutant of c-H-Ras, and Raf-1s621A, a dominant negative mutant of Raf-1, suggesting that Cot functions upstream of Ras and Raf-1.Other studies have shown that a kinase-negative, dominant negative mutant of Cot partially blocks Ras or Raf-1-induced MAP kinase activation, arguing that Cot functions downstream of Ras and Raf-1.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: COT (P284) polyclonal antibody detects endogenous levels of COT protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Cot (P284) pAb at 1:500 dilution
Lane 1: L02 whole cell lysate (40ug)
Lane 2: SGC7901 whole cell lysate (40ug)
Lane 3: PC3 whole cell lysate (40ug)
Lane 4: H9C2 whole cell lysate (40ug)
Lane 5: AML12 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of COT (P284) pAb in paraffin-embedded human brain tissue.

Bioworld Molecular Weight: ~ 53 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Mitogen-activated protein kinase kinase kinase 8; Cancer Osaka thyroid oncogene; Proto-oncogene c-Cot; Serine/threonine-protein kinase cot; Tumor progression locus 2; TPL-2; MAP3K8; COT; ESTF

Immunogen: Synthetic peptide, corresponding to amino acids 250-300 of Human Cot.

Conjugate: Unconjugated

Modification: Unmodified