Product Description

COTL1 Polyclonal Antibody

Catalogue Numbers: AP6021-50, AP6021-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q14019

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB:1:500~1:1000
IHC:1:50~1:200

Background: COTL1 (coactosin-like 1), also known as CLP, is a 142 amino acid cytoplasmic and cytoskeletal protein that belongs to the actin-binding proteins ADF family and coactosin subfamily. Widely expressed, COTL1 was first identified in slime mold and is found at highest levels in kidney, placenta, lung and peripheral blood leukocytes, with low levels found in pancreas, liver and brain. COTL1 is one of many proteins that participate in regulation of Actin’s cytoskeleton through a calcium dependent mechanism, but has not been shown to have a direct effect on Actin depolymerization. COTL1 contains one ADF-H domain and interacts with 5-LO (5-lipoxygenase), an enzyme that plays a role in the biosynthesis of leukotriene and is expressed in multiple types of leukocytes.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 96% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: COTL1 polyclonal antibody detects endogenous levels of COTL1 protein.

Extra Notes: Western blot (WB) analysis of COTL1 polyclonal antibody at 1:500 dilution
Lane1:HEK293T whole cell lysate(40ug)
Lane2:A549 whole cell lysate(40ug)
Lane3:The lung tissue lysate of Mouse(30ug)
Lane4:The lung tissue lysate of Rat(30ug)
Lane5:The kidney tissue lysate of Mouse(30ug)
Lane6:The kidney tissue lysate of Rat(30ug)

Bioworld Molecular Weight: ~ 16 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Coactosin-like protein; COTL1; CLP

Immunogen: Recombinant full length Human COTL1.

Conjugate: Unconjugated

Modification: Unmodified