COVID-19 Spike glycoProtein Polyclonal Antibody-BS67277

COVID-19 Spike glycoProtein Polyclonal Antibody

Catalogue Numbers: BS67277-50, BS67277-100

Sizes: 50µl, 100µl

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: P0DTC2

Host: Rabbit

Reactivity: COVID-19

Applications: ELISA, WB

All Applications: WB (1/1000 - 1/3000), ELISA: Use at an assay dependent dilution

Background: The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses. The genome of SARS-CoV-2 is similar to other coronaviruses, and is comprised of four key structural proteins: S, the spike protein, E, the envelope protein, M, the membrane protein, and N, the nucleocapsid protein. Coronavirus spike proteins are class I fusion proteins and harbor an ectodomain, a transmembrane domain, and an intracellular tail. The highly glycosylated ectodomain projects from the viral envelope surface and facilitates attachment and fusion with the host cell plasma membrane. The ectodomain can be further subdivided into host receptor-binding domain (RBD) (S1) and membrane-fusion (S2) subunits, which are produced upon proteolysis by host proteases at S1/S2 and S2’ sites. S1 and S2 subunits remain associated after cleavage and assemble into crown-like homotrimers. In humans, both SARS-CoV and SARS-CoV-2 spike proteins utilize the angiotensin-converting enzyme 2 (ACE2) protein as a receptor for cellular entry. Spike protein subunits represent a key antigenic feature of coronavirus virions, and therefore represent an important target of vaccines, novel therapeutic antibodies, and small-molecule inhibitors.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes COVID-19 Spike glycoprotein.

Extra Notes: Western blot analysis of COVID-19 Spike glycoprotein using Recombinant COVID-19 S Protein RBD-SD1 (A).

Bioworld Molecular Weight: ~ 40 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Spike glycoprotein; S glycoprotein; E2; Peplomer protein

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of COVID-19 Spike glycoprotein. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified