Product Description

COX1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90325-50, BS90325-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P23219(Human) P22437(Mouse) Q63921(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IHC FC IP,

All Applications: WB:1:1,000-1:5,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100

Background: Cytochrome c oxidase subunit I, COX1 (also designated COI, MTCO1 or oxidative phosphorylation (OxPhos) Complex IV, subunit I) is one of three mitochondrial DNA (mtDNA) encoded subunits (MTCO1-3) of respiratory Complex IV. Cytochrome c oxidase is a hetero-oligomeric enzyme composed of 13 subunits localized to the mitochondrial inner membrane and is the terminal enzyme complex of the electron transport chain. Complex IV catalyzes the reduction of molecular oxygen to water. The energy released is used to transport protons across the mitochondrial inner membrane. The resulting electro-chemical gradient is necessary for the synthesis of ATP. Complex IV contains 13 polypeptides; COX1, COX2 and COX3 (MTCO1-3) make up the catalytic core and are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc and VIII are nuclear-encoded.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: COX1 polyclonal antibody detects endogenous levels of COX1 protein.

Bioworld Molecular Weight: 69 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of COX1 on different lysates using anti-COX1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: C2C12
Lane 2: A431, ICC staining COX1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: COX-1, PGH synthase 1, PGHS-1, PHS 1, PTGS1, COX 1, COX 3, COX-1, COX1, Cox3, Cyclooxygenase 1, Cyclooxygenase 3, included, Cyclooxygenase-1, EC 1.14.99.1, Partial COX1 proteins, included, PCOX1, PGG/HS, PGH synthase 1, PGH1, PGHS-1, PGHS1, PHS 1, PHS1, Prostaglandin G/H synthase 1, Prostaglandin H2 synthase 1, Prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase), Prostaglandin-endoperoxide synthase 1, PTGHS, PTGS1,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified