COX5A Polyclonal Antibody-BS5674

COX5A Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS5674-50, BS5674-100

Sizes: 50µl, 100µl

Swiss-Prot: P20674

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: The cytochrome c oxidase (COX) family of proteins function as the final electron donor in the respiratory chain to drive a proton gradient across the inner mitochondrial membrane, ultimately resulting in the production of water. The mammalian COX apoenzyme is a dimer, with each monomer consisting of 13 subunits, some of which are mitochondrial and some of which are nuclear. Found in the inner mitochondrial membrane, COX5a, one of two subunits of the COX5 protein, is the heme A-containing chain of the oxidase family that converts one molecule of oxygen and four molecules of hydrogen to two molecules of water. When oxygen levels within the cell are high, transcription of COX5a, the aerobic subunit of the COX5 protein, is up-regulated as the rate of cellular respiration increases. Conversely, when oxygen levels are low, COX5a transcription decreases as the cell works to conserve oxygen by slowing the creation of water.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: COX5A polyclonal antibody detects endogenous levels of COX5A protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of COX5A polyclonal antibody at 1:500 dilution
Lane 1: MCF-7 cell lysate
Lane 2: sp2/0 cell lysate
Lane 3: PC12 cell lysate, Immunohistochemistry (IHC) analyzes of COX5A pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing Mitochondrion inner membrane staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 17 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Cytochrome c oxidase subunit 5A, mitochondrial; Cytochrome c oxidase polypeptide Va; COX5a; COX-5A

Immunogen: Synthetic peptide, corresponding to amino acids 53-98 of Human COX5A.

Conjugate: Unconjugated

Modification: Unmodified