Product Description

COXI Polyclonal Antibody

Catalogue Numbers: BS1636-50, BS1636-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9MD68

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:2000

Background: Cytochrome c oxidase subunit I, COX1 (also designated COI, MTCO1 or oxidative phosphorylation (OxPhos) Complex IV, subunit I) is one of three mitochondrial DNA (mtDNA) encoded subunits (MTCO1-3) of respiratory Complex IV. Cytochrome c oxidase is a hetero-oligomeric enzyme composed of 13 subunits localized to the mitochondrial inner membrane and is the terminal enzyme complex of the electron transport chain. Complex IV catalyzes the reduction of molecular oxygen to water. The energy released is used to transport protons across the mitochondrial inner membrane. The resulting electro-chemical gradient is necessary for the synthesis of ATP. Complex IV contains 13 polypeptides; COX1, COX2 and COX3 (MTCO1-3) make up the catalytic core and are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc and VIII are nuclear-encoded.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: COXI polyclonal antibody detects endogenous levels of COXI protein.

Extra Notes: Western blot (WB) analysis of COXI pAb at 1:2000 dilution
Lane1:The Brain tissue lysate of Mouse(40ug)
Lane2:The Skin tissue lysate of Rat(40ug)
Lane3:CT26 whole cell lysate(40ug)
Lane4:C6 whole cell lysate(40ug)
Lane5:Hela whole cell lysate(40ug)
Lane6:A375 whole cell lysate(30ug)

Bioworld Molecular Weight: ~ 57 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Cytochrome c oxidase subunit 1; Cytochrome c oxidase subunit I; Cytochrome oxidase subunit 1; mt-Co1; Co1; COX1; COXI

Immunogen: A synthetic peptide corresponding to amino acids 433-445 of Mouse COXI.

Conjugate: Unconjugated

Modification: Unmodified