COXIII Polyclonal Antibody-BS1674

COXIII Polyclonal Antibody

Catalogue Numbers: BS1674-50, BS1674-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q7JCX7

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: Cytochrome c oxidase subunit III, COX3 (also designated COIII, MTCO3 or oxidative phosphorylation (OxPhos) Complex IV, subunit III) is one of three mitochondrial DNA (mtDNA) encoded subunits (MTCO1-3) of respiratory Complex IV. Cytochrome c oxidase is a hetero-oligomeric enzyme composed of 13 subunits localized to the mitochondrial inner membrane and is the terminal enzyme complex of the electron transport chain. Complex IV catalyzes the reduction of molecular oxygen to water. The energy released is used to transport protons across the mitochondrial inner membrane. The resulting electro-chemical gradient is necessary for the synthesis of ATP. Complex IV contains 13 polypeptides; COX1, COX2 and COX3 (MTCO1-3) make up the catalytic core and are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc and VIII are nuclear-encoded. Apoptosis in mammalian cells may be triggered by inhibition of mitochondrial COX. Specifically, reduction of COX3 has been associated with apoptosis and studies have shown that overexpression of COX3 results in a reduction of signals associated with apoptosis.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: COXIII polyclonal antibody detects endogenous levels of COXIII protein.

Extra Notes: Western blot (WB) analysis of COXIII polyclonal antibody at 1:1000 dilution
Lane1:HepG-2 whole cell lysate(50μg)
Lane2:A549 whole cell lysate(57μg)
Lane3:The muscle tissue lysate of Mouse (33μg)

Bioworld Molecular Weight: ~ 30 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Cytochrome c oxidase subunit 3; Cytochrome c oxidase subunit III; Cytochrome c oxidase sununit III; Cytochrome oxidase sununit 3; mt-Co3; Co3; COIII; COX3; COXIII

Immunogen: A synthetic peptide corresponding to amino acids 57-71 of Mouse COXIII .

Conjugate: Unconjugated

Modification: Unmodified