CPI-17 (phospho-T38) Polyclonal Antibody-BS4780

CPI-17 (phospho-T38) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS4780-50, BS4780-100

Swiss-Prot: Q96A00

Host: Rabbit

CPI-17 (phospho-T38) Polyclonal Antibody-BS4780

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: CPI-17 is a phosphorylation-dependent inhibitory protein for smooth muscle myosin phosphate. CPI-17 was originally identified as a PKC-potentiated inhibitory protein of protein phosphatase-1, which is dominantly expressed in smooth muscle. Phosphorylation at Threonine 38, in vitro, by PKC or Rho-kinase enhances the inhibitory potency toward myosin phosphatase. CPI-17 is also phosphorylated at Threonine 38 by protein kinase N and might be involved in the calcium sensitization of smooth muscle contraction as a downstream effector of Rho and/or arachidonic acid. CPI-17 is dually phosphorylated at Serine 12 and Threonine 38 by a MYPT-associated kinase, M110 kinase.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-CPI-17 (T38) polyclonal antibody detects endogenous levels of CPI-17 polyclonal antibody protein only when phosphorylated at Thr38.

Bioworld Molecular Weight: ~ 22 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of p-CPI-17 (T38) pAb at 1:500 dilution
Lane 1: A549 whole cell lysate (40ug)
Lane 2: HEK293T whole cell lysate (40ug)
Lane 3: PC12 whole cell lysate (40ug)
Lane 4: The Brain tissue lysate of Mouse (20ug), Immunohistochemistry (IHC) analyzes of p-CPI-17 (T38) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.

Alternative Name: Protein phosphatase 1 regulatory subunit 14A; 17 kDa PKC-potentiated inhibitory protein of PP1; Protein kinase C-potentiated inhibitor protein of 17 kDa; CPI-17; CPI17; PPP1INL; PPP1R14A

Immunogen: Synthetic phosphopeptide derived from human CPI-17 around the phosphorylation site of Threonine 38.

Modification: Phosphorylation

Conjugate: Unconjugated