CPLX3 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS75416-50, BS75416-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q8WVH0
Host: Rabbit
Reactivity: Mouse, Rat
Applications: WB
All Applications: WB,1:500 - 1:2000
Background: Complexin that regulates SNARE protein complex-mediated synaptic vesicle fusion (By similarity. Required for the maintenance of synaptic ultrastructure in the adult retina (By similarity. Positively regulates synaptic transmission through synaptic vesicle availability and exocytosis of neurotransmitters at photoreceptor ribbon synapses in the retina (By similarity. Suppresses tonic photoreceptor activity and baseline 'noise' by suppression of Ca(2+ vesicle tonic release and the facilitation of evoked synchronous and asynchronous Ca(2+ vesicle release (By similarity.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Unmodification
Extra Notes: Western blot analysis of extracts of various cell lines, using CPLX3 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Note: For research use only, not for use in diagnostic procedure.
Bioworld Molecular Weight: 18kDa
Alternative Name: CPLX3; CPX-III; CPXIII; Nbla11589
Immunogen: Recombinant fusion protein of human CPLX3(NP_001025176.1).
Conjugate: Unconjugated
Modification: Unmodified