Product Description

CPN2 Polyclonal Antibody

Catalogue Numbers: BS5676-50, BS5676-100

Sizes: 50µl, 100µl

Alternative Name: Carboxypeptidase N subunit 2; Carboxypeptidase N 83 kDa chain; Carboxypeptidase N large subunit; Carboxypeptidase N polypeptide 2; Carboxypeptidase N regulatory subunit; ACBP; CPN reg; CPN-reg; CPNreg;

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P22792

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Carboxypeptidase N (arginine carboxypeptidase or CPN) cleaves basic amino acid residues from the carboxy terminal of peptides and proteins. The enzyme plays a central role in regulating the biologic activity of peptides such as kinins and anaphylatoxins, and therefore is also known as kininase-1 and anaphylatoxin inactivator. CPN is a tetrameric complex consisting of two identical regulatory subunits (CPN reg) and two identical catalytic subunits (CPN cat). The two glycosylated CPN reg subunits protect the two CPN cat subunits and keep them in the circulation. CPN reg is a member of the leucinerich repeat family of proteins and the gene which encodes CPN reg maps to human chromosome 8p22-p23. CPN cat is a member of the regulatory B-type carboxypeptidase group and the gene which encodes CPN cat maps to human chromosome 10.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CPN2 polyclonal antibody detects endogenous levels of Carboxypeptidase N regulatory subunit protein.

Bioworld Molecular Weight: ~ 61, 83 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CPN2 polyclonal antibody at 1:500 dilution
Lane 1: MCF-7 cell lysate
Lane 2: Hela cell lysate
Lane 3: Raw264.7 cell lysate

Immunogen: Synthetic peptide, corresponding to the 490-535 of Human CPN2.

Conjugate: Unconjugated

Modification: Unmodified