Product Description

CPS1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90328-50, BS90328-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P31327(Human)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IF, IHC, IP,

All Applications: WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200

Background: The multicomplex protein, carbamoyl-phosphate synthetase-aspartate carbamoyl transferase-dihydro-orotase (CAD), consists of three distinct proteins, carbamoyl phosphate synthetase 2 (CPS2), aspartate transcarbamylase, and dihydro-orotase, which catalyze the second and third steps of pyrimidine biosynthesis. CAD is allosterically regulated by the phosphorylation of CPS2 by cyclic AMP-dependent protein kinase, and this activation enables CPS2 to catalyze the rate-limiting step of pyrimidine synthesis. CAD is expressed in brain and skeletal muscle. A related protein, carbamoyl phosphate synthetase 1 (CPS1) is expressed in liver. CPS1 catalyzes the rate-limiting step in the urea cycle, and deficiency of CPS1 is an autosomal recessive disorder that causes hyperammonemia.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: CPS1 polyclonal antibody detects endogenous levels of CPS1 protein.

Bioworld Molecular Weight: 165 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of CPS1 on SiHa cell using anti-CPS1 antibody at 1/500 dilution., ICC staining CPS1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: CPSase I, CPS1, Carbamoyl phosphate synthase [ammonia], Carbamoyl phosphate synthase [ammonia] mitochondrial, Carbamoyl phosphate synthase, Carbamoyl phosphate synthetase 1, Carbamoyl phosphate synthetase 1 mitochondrial, Carbamoyl phosphate synthetase I, Carbamoyl-phosphate synthase [ammonia], Carbamoyl-phosphate synthetase I, Carbamoylphosphate synthase, Carbamoylphosphate synthetase 1, Carbamoylphosphate synthetase I, CPS 1, Cps1, CPSase 1, CPSase I, CPSASE1, CPSM, mitochondrial, MS738,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified