Product Description

CPT2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90329-50, BS90329-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P23786(Human) P52825(Mouse) P18886(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC

All Applications: WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200

Background: The mitochondrial β-oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase (CPT) I (outer membrane and detergent labile) and II (inner membrane and detergent stable), together with carnitine carrier. CPTI catalyzes the first reaction in the transport of long-chain fatty acids from the cytoplasm to the mitochondrion, a rate-limiting step in beta-oxidation. Two types of CPTI are known, the liver (CPTIA) and muscle (CPTIB) isoforms. The muscle type protein is specially expressed in heart and skeletal muscle. Membrane-bound CPTI, but not CPTII, is inhibited reversibly by malonyl-coenzyme A (CoA). Unlike CPTII, CPTI requires membrane integrity for catalytic function. In addition, glutamic acid 3 and histidine 5 are necessary for malonyl CoA inhibition and binding to liver CPTI, but not for catalytic activity.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: CPT2 polyclonal antibody detects endogenous levels of CPT2 protein.

Bioworld Molecular Weight: 74 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of CPT2 on different lysates using anti-CPT2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: 293
Lane 3: HepG2
Lane 4: NIH/3T3, ICC staining CPT2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: CPT II, CPT2, Carnitine O palmitoyltransferase 2, Carnitine O palmitoyltransferase 2 mitochondrial, Carnitine O-palmitoyltransferase 2, Carnitine palmitoyltransferase 2, Carnitine palmitoyltransferase II, CPT 1, CPT 2, CPT II, CPT1, CPT2, CPT2, CPTASE, CPTII, IIAE4, mitochondrial,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified