CRABP-II (P81) Polyclonal Antibody-BS3279

CRABP-II (P81) Polyclonal Antibody

Catalogue Numbers: BS3279-50, BS3279-100

Sizes: 50µl, 100µl

Alternative Name: Cellular retinoic acid-binding protein 2; Cellular retinoic acid-binding protein II; CRABPII; CRABP II; CRABP2; CRABP 2

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P29373

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The cellular retinoic acid-binding protein (CRABP)-I and a related isoform CRABP-II are nuclear receptors for retinoic acid (RA), an important regulator of cell growth and differentiation in fetal and adult tissues. These CRABP proteins mediate the downstream effects of RA in distinct ways. CRABP-I negatively regulates the activity of RA by enhancing the production of RAmetabolizing enzymes and increasing the rate at which RA is degraded. CRABP-II enhances the effects of RA by directly interacting with RA receptors (RAR) and, in turn, promoting the formation of RAR-RA complexes and stimulating RA-mediated gene transcription. Both CRABP-I and CRABP-II are expressed in the embryo, and CRABP-I is ubiquitously expressed in various adult tissues. The expression of CRABP-II is elevated in cells that synthesize relatively large amounts of RA, and it is also predominantly expressed in skin, uterus, ovary, and in the choroid plexus.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CRABP-II (P81) polyclonal antibody detects endogenous levels of CRABP-II cat protein.

Bioworld Molecular Weight: ~ 16 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CRABP-II (P81) pAb at 1:500 dilution
Lane 1: PC3 whole cell lysate(40ug)
Lane 2: C6 whole cell lysate(40ug)
Lane 3: 3T3-L1 whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 50-100 of Human NCRABP-II.

Conjugate: Unconjugated

Modification: Unmodified