CREB Rabbit Monoclonal Antibody-BS9811M

CREB Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9811M-50, BS9811M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P16220

Host: Rabbit

Reactivity: Human, Mouse, Rat, Zebra fish

Applications: WB, ICC/IF, IHC, IP, , FC

All Applications: WB: 1:1000-1:5000 IHC/ICC/IF: 1:50-1:200
IP: 1:50-1:200 FC: 1:10-1:100

Background: Cyclic AMP Response Element Binding protein (CREB) is a basic / leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation occurs via p44 / 42 MAP kinase and p90RSK and also via p38 MAP kinase and MSK 1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of CREB and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 38 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CREB Rabbit mAb at 1:1000 dilution
Lane 1: Hela whole cell lysate
Lane 2: HepG2 whole cell lysate
Lane 3: Jurkat whole cell lysate
Lane 4: NIH/3T3 whole cell lysate, Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CREB antibody. Counter stained with hematoxylin., ICC staining CREB in Hela cells (green). The nuclear counter stain is DAPI (blue).
Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.

Alternative Name: Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1; CREB1

Immunogen: Recombinant antibody.

Conjugate: Unconjugated

Modification: Unmodified