Product Description

CREB1 Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS79603-50, BS79603-100

Swiss-Prot: P16220

Host: Rabbit

CREB1 Polyclonal Antibody-BS79603

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IP,

All Applications: WB,1:500 - 1:1000 | IP,1:50 - 1:200

Background: This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Unmodification

Bioworld Molecular Weight: 43kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using CREB1 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST., Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug CREB1 antibody. Western blot was performed from the immunoprecipitate using CREB1 antibody at a dilution of 1:1000.

Alternative Name: CREB1; CREB; CREB-1

Immunogen: Recombinant fusion protein of human CREB1(NP_604391.1).

Modification: Unmodified

Conjugate: Unconjugated