Product Description

CRF-RI (H177) Polyclonal Antibody

Catalogue Numbers: BS2590-50, BS2590-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P34998

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB IHC IF

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Background: A high affinity CRF binding protein, designated CRF-BP, has been discovered in postmortem brain samples from AD patients. CRF-BP serves to bind and inactivate CRF, reducing the pool of “free CRF” available to bind CRFRs. Two CRF receptors, designated CRF-RI and CFR-RII, exhibit distinct brain localizations.Two forms of CFR-RII, designated CFR-RIIα and CFR-RIIβ, result from alternative mRNA splicing. Urocortin, an additional member of the CRF family, shares 63% sequence identity with urotensin and 45% sequence identity with CRF. Urocortin specifically binds to and activates CRF-RI and CRF-RII, but binds to CRF-RII more efficiently than CRF, suggesting that it may be the true, high affinity ligand for the CRF receptor type II.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CRF-RI (H177) polyclonal antibody detects endogenous levels of CRF-RI protein.

Extra Notes: Western blot (WB) analysis of CRF-RI (H177) pAb at 1:1000 dilution
Lane1:L02 whole cell lysate(20ug)
Lane2:MCF-7 whole cell lysate(20ug)
Lane3:A549 whole cell lysate(40ug)
Lane4:C6 whole cell lysate(40ug)
Lane5:BV2 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 50 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Corticotropin-releasing factor receptor 1; CRF-R-1; CRF-R1; CRFR-1; Corticotropin-releasing hormone receptor 1; CRH-R-1; CRH-R1; CRHR1; CRFR; CRFR1; CRHR

Immunogen: Synthetic peptide, corresponding to amino acids 150-200 of Human CRF-RI.

Conjugate: Unconjugated

Modification: Unmodified