Product Description

CRF-RII (H112) Polyclonal Antibody

Catalogue Numbers: BS2616-50, BS2616-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13324

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF

All Applications: WB: 1:500~1:1000
IF: 1:50~1:200

Background: Two CRF receptors, designated CRF-RI and CFR-RII, exhibit distinct brain localizations.Two forms of CFR-RII, designated CFR-RIIα and CFR-RIIβ, result from alternative mRNA splicing. Urocortin, an additional member of the CRF family, shares 63% sequence identity with urotensin and 45% sequence identity with CRF. Urocortin specifically binds to and activates CRF-RI and CRF-RII, but binds to CRF-RII more efficiently than CRF, suggesting that it may be the true, high affinity ligand for the CRF receptor type II.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CRF-RII (H112) polyclonal antibody detects endogenous levels of CRF-RII protein.

Extra Notes: Western blot (WB) analysis of CRF-RII (H112) polyclonal antibody at 1:500 dilution
Lane1:Hela cell lysate
Lane2:sp2/0 cell lysate
Lane3:H9C2 cell lysate

Bioworld Molecular Weight: ~ 48 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Corticotropin-releasing factor receptor 2; CRF-R-2; CRF-R2; CRFR-2; Corticotropin-releasing hormone receptor 2; CRH-R-2; CRH-R2; CRF2R; CRH2R; CRHR2

Immunogen: Synthetic peptide, corresponding to amino acids 96-85 of Human CRF-RII.

Conjugate: Unconjugated

Modification: Unmodified