Product Description

CRHBP Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS6948-50, BS6948-100

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Swiss-Prot: P24387

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB ELISA IF

All Applications: WB: 1:500-1:2000
ELISA: 1:5000-1:10000
IF: 1:50-1:200

Background: Response to stress in mammals requires an intact hypothalamic-pituitary-adrenal axis. The proximal part of the response is mediated by secretion of corticotropin-releasing hormone (CRH) by the paraventricular nucleus of the hypothalamus. CRH is a 41 amino acid peptide derived by enzymatic cleavage from a 191 amino acid preprohormone. CRH is produced not only in the hypothalamus but also in peripheral tissues, such as T lymphocytes; it is highly expressed in human placenta. Glucocorticoids stimulate placental CRH synthesis and secretion in primary cultures of human placenta. This stimulation is in contrast to the glucocorticoid suppression of CRH expression in hypothalamus. The gene which encodes CRH maps to human chromosome 8q13. Human plasma contains a CRH-binding protein, CRH-BP (also designated CRF-BP) which inactivates CRH and which may prevent inappropriate pituitary-adrenal stimulation in pregnancy. The gene which encodes CRF-BP maps to human chromosome 5q11.2-q13.3 .

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CRHBP polyclonal antibody detects endogenous levels of CRHBP protein.

Bioworld Molecular Weight: ~ 45 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Primary: Anti-CRHBP at 1/1000 dilution

Alternative Name: Corticotropin-releasing factor-binding protein; CRF-BP; CRF-binding protein; Corticotropin-releasing hormone-binding protein; CRH-BP; CRHBP; CRFBP

Immunogen: KLH conjugated synthetic peptide derived from human CRHBP: 71-170/322

Conjugate: Unconjugated

Modification: Unmodified