CRISPR-Cas9 Mouse Monoclonal Antibody-MB9030

CRISPR-Cas9 Mouse Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB9030-50, MB9030-100

Product: Mouse IgM, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q99ZW2

Host: Mouse

Reactivity: Recombinant Cas9

Applications: WB, ICC

All Applications: WB: 1:1000-5000
ICC: 1:50-200

Background: CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects recombinant Cas9 protein.

Bioworld Molecular Weight: ~ 158 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CRISPR/Cas9 Mouse mAb at 1:5000 dilution
Lane 1: CRISPR/Cas9 transfected 293 cells lysate
Lane 2: Non-transfected 293 cells lysate, Immunofluorescence staining CRISPR/Cas9 in CRISPR/Cas9 transfected 293T cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.

Alternative Name: Cas9; CRISPR-associated endonuclease Cas9/Csn1; CRISPR-Cas9/Csn1; csn1; SpyCas9

Immunogen: This antibody is produced by immunizing mice with a recombinant protein corresponding to a sequence of CRISPR/Cas9.

Conjugate: Unconjugated

Modification: Unmodified