Product Description

CRISPR-Cas9 SP Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90331-50, BS90331-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q99ZW2

Host: Rabbit

Reactivity: Recombinant Cas9

Applications: WB

All Applications: WB: 1:500~1:1000

Background: CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CRISPR/Cas9 (R780) polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate.
Lane 2: HEK293T transfected with CRISPR/Cas9 (L:P=3:1).
Lane 3: HEK293T transfected with CRISPR/Cas9 (L:P=6:1).
Lane 4: HEK293T transfected with CRISPR/Cas9 (L:P=9:1).

Bioworld Molecular Weight: ~ 160 kDa

Specificity: CRISPR-Cas9 SP polyclonal antibody detects endogenous levels of CRISPR-Cas9 SP protein.

Alternative Name: Cas9; CRISPR-associated endonuclease Cas9/Csn1; CRISPR-Cas9/Csn1; csn1; SpyCas9

Immunogen: Synthetic peptide, corresponding to amino acids 750-800 of CRISPR/Cas9.

Conjugate: Unconjugated

Modification: Unmodified