Crk2 Monoclonal Antibody-MB0130

Crk2 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB0130-50, MB0130-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P46108

Host: Mouse

Reactivity: Human

Applications: WB

All Applications: WB: 1:1000

Background: Crk I and Crk II are encoded by the same gene, which undergoes alternative splicing to yield these two proteins, but differ in their biological activities. Crk II has less transforming activity than Crk I, although both Crk I and Crk II bind to many tyrosine-phosphorylated proteins that bind to GRB2. In addition, Crk II becomes rapidly tyrosine-phosphorylated in response to stimulation with insulin-like growth factor I (IGF-I) and might be involved in the IGF-I receptor signalling pathway.

Purification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Crk2 mAb detects endogenous levels of Crk2 protein.

Bioworld Molecular Weight: Predicted band size:34kDa
Observed band size:34kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western BlotDetection of CRK in LNCAP, A431, MCF7&HT1080 cell lysates using CRK antibody (1:1000 diluted).

Alternative Name: Adapter molecule crk; Proto-oncogene c-Crk; p38 CRK; CRK; CRKII

Immunogen: Recombinant full length Human Crk2.

Conjugate: Unconjugated

Modification: Unmodified