Product Description

Crk2 (phospho-Try221) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS5064-50, BS5064-100

Sizes: 50µl, 100µl

Swiss-Prot: P46108

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: IHC

Background: Crk I and Crk II are encoded by the same gene, which undergoes alternative splicing to yield these two proteins, but differ in their biological activities. Crk II has less transforming activity than Crk I, although both Crk I and Crk II bind to many tyrosine-phosphorylated proteins that bind to GRB2. In addition, Crk II becomes rapidly tyrosine-phosphorylated in response to stimulation with insulin-like growth factor I (IGF-I) and might be involved in the IGF-I receptor signalling pathway.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-Crk2 (Try221) polyclonal antibody detects endogenous levels of Crk2 protein only when phosphorylated at Try221.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry (IHC) analyzes of p-Crk2 (Try221) pAb in paraffin-embedded human liver carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 42 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Adapter molecule crk; Proto-oncogene c-Crk; p38 CRK; CRK; CRKII

Immunogen: Synthetic phosphopeptide derived from human Crk2 around the phosphorylation site of Tyrosine 221.

Conjugate: Unconjugated

Modification: Phosphorylation