Product Description

CrkL (phospho-Y207) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS4056-50, BS4056-100

Swiss-Prot: P46109

Host: Rabbit

CrkL (phospho-Y207) Polyclonal Antibody-BS4056

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: Crk, is a transformation-specific protein that induces elevation of cellular phosphotyrosine levels, but lacks tyrosine kinase activity itself. A second protein, Nck, consists solely of three SH3 domains and one SH2 domain, while GRB2 contains an SH2 domain flanked on both sides by SH3 domains. A member of this protein class, Crk-L, is encoded by a gene located on chromosome 22, band 11, centromeric of the chronic myelogenous leukemia breakpoint region. Crk-L encodes a 303 amino acid protein with one SH2 and two SH3 domains, and a predicted molecular weight of 36 kDa.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-Crk-L (Y207) polyclonal antibody detects endogenous levels of Crk-L protein only when phosphorylated at Tyr207.

Bioworld Molecular Weight: ~ 39 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of p-CrkL (Y207) pAb at 1:500 dilution
Lane 1: MCF-7 whole cell lysate (40ug)
Lane 2: HEK293T whole cell lysate (40ug)
Lane 3: PMVEC whole cell lysate (40ug)
Lane 4: CT26 whole cell lysate (40ug)
Lane 5: SGC7901 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of p-Crk-L (Y207) pAb in paraffin-embedded human colon cancer tissue.

Alternative Name: Crk L; Crk like protein; Crk-like protein; Crkl; Crkol; Oncogene CrkL;

Immunogen: Synthetic phosphopeptide derived from human CrkL around the phosphorylation site of Tyrosine 207.

Modification: Phosphorylation

Conjugate: Unconjugated