Product Description

CRY1 Polyclonal Antibody

Catalogue Numbers: GCP94-50, GCP94-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q16526

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB: 1:1000~1:2000

Background: Circadian Clocks are biological timepieces that regulate hormonal rhythms, sleep cycles and feeding behaviors. These rhythms are generated in the superchiasmatic nucleus (SCN), a cell-autonomous circadian oscillator located within the brain that is synchronized with the environment by light. A number of transcription factors, including Clock and BMAL1, are molecular components of the SCN that induce the expression of proteins involved in light/dark cycle entrainment, which include Per1 and Per2. Tim, for timeless, generates a negative feedback loop that regulates the activity of Clock by suppressing the expression of Clock target genes. Tim forms heterodimers with Per1 and Per2 that bind Clock and block the activation of Clock-BMAL1 dimers to repress Per gene expression. Additionally, the CRY proteins, which are cryptochrome photoreceptors for the circadian Clock, function as light-independent inhibitors of the circadian Clock. CRY1 and CRY2 negatively regulate SCN components by associating with the activators Clock-BMAL1, and also with the various feedback inhibitors Per1, Per2 and Tim.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CRY1 polyclonal antibody detects endogenous levels of CRY1 protein.

Extra Notes: Western blot (WB) analysis of CRY1 polyclonal antibody at 1:1000 dilution
Lane1:HepG2 whole cell lysate(40ug)
Lane2:HEK293T whole cell lysate(40ug)
Lane3:A549 whole cell lysate(40ug)
Lane4:MCF-7 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 68 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Cryptochrome-1; CRY1; PHLL1

Immunogen: Synthetic peptide, corresponding to Human CRY1.

Conjugate: Unconjugated

Modification: Unmodified