Product Description

Crystallin-αB (L55) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1626-50, BS1626-100

Sizes: 50µl, 100µl

Swiss-Prot: P02511

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: α-crystallins consist of three gene products, αA, αB and αC-crystallin, which are members of the small heat shock protein family (HSP 20). They are induced by heat shock, and act as molecular chaperones by holding denatured proteins in large soluble aggregates. However, unlike other molecular chaperones, α-crystallins do not renature these proteins. Expression of αA-crystallin is restricted to the lens. Defects in this gene cause autosomal dominant congenital cataracts (ADCC). The human αB-crystallin gene product is expressed in many tissues, including lens, heart and skeletal muscle. Elevated expression of αB-crystallin is associated with many neurological diseases, and a missense mutation in this gene has co-segregated in a family with a Desmin-related myopathy.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Crystallin-αB (L55) polyclonal antibody detects endogenous levels of Crystallin-αB protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Crystallin-αB (L55) pAb at 1:500 dilution
Lane 1: MEF whole cell lysate (40ug)
Lane 2: PMVEC whole cell lysate (40ug)
Lane 3: A549 whole cell lysate (40ug)
Lane 4: HEK293T whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of Crystallin-αB (L55) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 20 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Alpha-crystallin B chain; Alpha (B)-crystallin; Heat shock protein beta-5; HspB5; Renal carcinoma antigen NY-REN-27; Rosenthal fiber component; CRYAB; CRYA2; αB-Crystallin

Immunogen: Synthetic peptide, corresponding to amino acids 21-70 of Human Crystallin-αB.

Conjugate: Unconjugated

Modification: Unmodified