Crystallin αB Polyclonal Antibody-BS90335

Crystallin αB Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90335-50, BS90335-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P02511(Human) P23928(Rat)

Host: Rabbit

Reactivity: Human, Rat

Applications: WB, IHC ICC/IF, IP,

All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
IP:1:50

Background: Crystallins are the major proteins of the vertebrate eye lens, where they maintain the transparency and refractive index of the lens. Crystallins are divided into α, β and γ families, and the β- and γ-crystallins also compose a superfamily. Crystallins usually contain seven distinct protein regions, inclu-ding four homologous motifs, a connecting peptide, and N- and C-terminal extensions. α-crystallins consist of three gene products, αA-, αB- and αC-crystallin, which are members of the small heat shock protein family (HSP 20). α-crystallins act as molecular chaperones by holding denatured proteins in large soluble aggregates. However, unlike other molecular chaperones, α-crystallins do not renature these proteins. Expression of αA-crystallin is restricted to the lens and defects of this gene cause the development of autosomal dominant congenital cataracts (ADCC). The human αB-crystallin gene product is expressed in many tissues, including lens, heart and skeletal muscle. Elevated expression of αB-crystallin is associated with many neurological diseases, and a missense mutation in this gene has co-segregated in a family with a Desmin-related myopathy.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Crystallin αB polyclonal antibody detects endogenous levels of Crystallin αB protein.

Bioworld Molecular Weight: 20 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Alpha B Crystallin on human skeleton musle (1) and mouse heart (2) tissues lysate using anti-Alpha B Crystallin antibody at 1/1,000 dilution., ICC staining Alpha B Crystallin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: HspB5, CRYAB, CRYA2, HSPB5, AACRYA, Alpha B crystallin, Alpha crystallin B chain, Alpha(B)-crystallin, Alpha-crystallin B chain, CRYA2, Cryab, CRYAB, Crystallin alpha B, Crystallin alpha polypeptide 2, CTPP2, Heat shock 20 kD like protein, Heat shock protein beta 5, Heat shock protein beta-5, HspB5, Renal carcinoma antigen NY REN 27, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified