CSF3 Polyclonal Antibody
Catalogue Numbers: BS60536-50, BS60536-100
Sizes: 50µl, 100µl
Alternative Name: Granulocyte colony-stimulating factor; G-CSF; Pluripoietin; INN: Filgrastim; INN: Lenograstim; CSF3; C17orf33; GCSF
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P09919
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: Granulocyte-colony stimulating factor, G-CSF, is a pleiotropic cytokine that influences differentiation, proliferation and activation of the neutrophilic granulocyte lineage. The murine G-CSF cDNA encodes a 208 amino acid precursor containing a 30 amino acid signal peptide that is proteolytically cleaved to form a 178 amino acid residue mature protein. Two G-CSF cDNAs, which are identical except for a three amino acid deletion in the N-terminus of one form of the protein, have been isolated from human cells. Murine and human G-CSF share 73% sequence identity at the amino acid level. G-CSF signals through the G-CSF receptor, G-CSFR, a heavily glycosylated 812 amino acid polypeptide with a single transmembrane domain. Stimulation of the G-CSFR results in the activation of the Ras/MAPK pathway and phosphorylation of the adaptor protein Shc. Studies indicate that the kinases Lyn and Syk and the transcription factor Stat3 are activated in response to G-CSF stimulation.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: CSF3 polyclonal antibody detects endogenous levels of CSF3 protein.
Bioworld Molecular Weight: ~ 22 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of CSF3 polyclonal antibody at 1:500 dilution
Lane 1: Hela whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: PC12 whole cell lysate
Immunogen: A synthetic peptide corresponding to residues in Human CSF3.
Conjugate: Unconjugated
Modification: Unmodified