Product Description

CSRP1 Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS5677-50, BS5677-100

Sizes: 50µl, 100µl

Swiss-Prot: P21291

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC ICC IP

Background: Cysteine-rich proteins (CRPs) participate in the organization of multiprotein complexes, both in the cytoplasm, where they participate in cytoskeletal remodeling, and in the nucleus, where they facilitate smooth muscle differentiation. CRP1 (cysteine and glycine-rich protein 1), also known as CRP, CSRP1 or CYRP, is abundant in the prostate and smooth muscle lineages. It contains two LIM zinc-binding domains and is localized in the nucleus. The LIM domains of CRP1 are critical for binding to the adhesion-plaque protein Zyxin. CRP1 also interacts with a-actinin to mediate muscle differentiation. These associations indicate that the main function of CRP1 may be structural.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: CSRP1 polyclonal antibody detects endogenous levels of CSRP1 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CSRP1 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T cell lysate
Lane 2: Mouse heart tissue lysate
Lane 3: Rat heart tissue lysate, Immunohistochemistry (IHC) analyzes of CSRP1 pAb in paraffin-embedded human esophageal carcinoma tissue at 1:50.showing nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 23 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Cysteine and glycine-rich protein 1; Cysteine-rich protein 1; CRP; CRP1; Epididymis luminal protein 141; HEL-141; HEL141; CSRP; CYRP

Immunogen: Synthetic peptide, corresponding to amino acids 88-133 of Human CSRP1.

Conjugate: Unconjugated

Modification: Unmodified