CtBP1 Polyclonal Antibody-BS90337

CtBP1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90337-50, BS90337-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13363(Human) Q9Z2F5(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC ICC IF, IP,

All Applications: WB:1:500-1:2,000
IHC:1:50-1:200
ICC:1:50-1:100
IP:1:10-1:50

Background: CtBP1 is a cellular phosphoprotein that associates with various proteins and functions as a corepressor of transcription. CtBP1 and the related protein CtBP2 are characterized as C-terminal binding protein of adenovirus E1A, and they preferentially associate with the E1A via a 5-amino acid motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation. CtBP1 is expressed from embryo to adult, but CtBP2 is mainly expressed during embryogenesis. During skeletal and T-cell development, CtBP1 and CtBP2 associate with the PLDLSL domain of δEF1, a cellular zinc finger-homeodomain protein, and thereby enhances δEF1 induced transcriptional silencing. In addition, CtBP complexes with CtIP, a protein that recognizes distinctly different protein motifs from CtBP. CtIP binds to the BRCT repeats within the breast cancer gene BRCA1 and enables CtBP to influence BRCA1 activity. CtIP/CtBP binding to BRCA1 inhibits the transactivation of the p21 promoter, and it is critical for regulating p21 transcription in response to DNA damage.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: CtBP1 polyclonal antibody detects endogenous levels of CtBP1 protein.

Bioworld Molecular Weight: 47 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of CtBP1 on PC-3M cell lysate using anti-CtBP1 antibody at 1/1,000 dilution., ICC staining CtBP1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: CtBP1, CTBP1, CTBP, BARS, brefeldin A- ribosylated substrate, C terminal binding protein 1, C-terminal-binding protein 1, CTBP, CtBP1, CTBP1, MGC104684,

Immunogen: Recombinant protein within human CtBP1 aa 300-500.

Conjugate: Unconjugated

Modification: Unmodified