CTBP2 Polyclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: BS76963-50, BS76963-100 Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Swiss-Prot: P56545 Host: Rabbit Reactivity: Human, Mouse, Rat Applications: WB, IF/ICC All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200 Background: This gene produces alternative transcripts encoding two distinct proteins. One protein is a transcriptional repressor, while the other isoform is a major component of specialized synapses known as synaptic ribbons. Both proteins contain a NAD+ binding domain similar to NAD+-dependent 2-hydroxyacid dehydrogenases. A portion of the 3' untranslated region was used to map this gene to chromosome 21q21.3; however, it was noted that similar loci elsewhere in the genome are likely. Blast analysis shows that this gene is present on chromosome 10. Several transcript variants encoding two different isoforms have been found for this gene. Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity: Unmodification Bioworld Molecular Weight: 49kDa Note: For research use only, not for use in diagnostic procedure. Extra Notes: Western blot analysis of extracts of various cell lines, using CTBP2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per Lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s., Immunofluorescence analysis of C6 cells using CTBP2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of U2OS cells using CTBP2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining. Alternative Name: CTBP2 Immunogen: A synthetic peptide of human CTBP2(NP_001320.1). Conjugate: Unconjugated Modification: Unmodified