CTCF Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90338-50, BS90338-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P49711(Human) Q61164(Mouse) Q9R1D1(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat, Zebrafish
Applications: WB, ICC/IF, IHC, IP,
All Applications: WB:1:500-1:1,000
ICC:1:100-1:500
IHC:1:50-1:200
IP:1:50-1:100
Background: CTCF belongs to the zinc finger transcription factor family, and it recognizes unusually long and remarkably divergent DNA target sequences to influence expression of many various genes. The DNA-binding domain of CTCF is composed of 11 Zn fingers including 10 that are of C2H2 class, and 1 that is of C2HC class, and they are highly conserved between vertebrate species. CTCF functions as a repressor of the c-myc gene and as a regulator of lysozyme gene expression. In addition, CTCF associates with the essential activator domain in the promotor region of the amyloid beta-protein precursor (APP) gene to activate transcription of APP. Expression of CTCF up-regulates APP expression and thereby, enhances synapse formations between primary neurons during development. CTCF is ubiquitously expressed and localized to the nucleus. During terminal differentiation, CTCF is negatively regulated by differential phosphorylation and also by decreases in CTCF mRNA and protein expression.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: CTCF polyclonal antibody detects endogenous levels of CTCF protein.
Bioworld Molecular Weight: 140/83 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of CTCF on different cell lysates using anti-CTCF antibody at 1/500 dilution.
Positive control:
Lane 1: 293T
Lane 2:MCF-7, ICC staining CTCF in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: CTCF, 11 zinc finger protein, 11 zinc finger transcriptional repressor, 11-zinc finger protein, CCCTC binding factor (zinc finger protein), CCCTC binding factor, CCCTC-binding factor, Ctcf, CTCF, CTCFL paralog, MRD21, Transcriptional repressor CTCF,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified