Product Description

CTDSP1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS61626-50, BS61626-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9GZU7

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IP,

All Applications: WB: 1:500~1:1000
IP: 1:50~1:200

Background: Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1, preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residue repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CTDSP1 polyclonal antibody detects endogenous levels of CTDSP1protein.

Bioworld Molecular Weight: ~ 29 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CTDSP1 polyclonal antibody at 1:500 dilution
Lane 1: NIH-3T3 whole cell lysate (40ug)
Lane 2: PMVEC whole cell lysate (40ug)
Lane 3: H1792 whole cell lysate (40ug)
Lane 4: A549 whole cell lysate (40ug), Immunoprecipitation of SGC7901 cell lysate using CTDSP1 polyclonal antibody (Sepharose Bead Conjugate) #BD0048 (Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) #BD0048 (Lane 4). Lane 1 is 30% input. The western blot was probed using CTDSP1 #BS61626. “↑”（supernatant）;“↓(deposition）

Alternative Name: Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1; Nuclear LIM interactor-interacting factor 3; NLI-IF; NLI-interacting factor 3; Small C-terminal domain phosphatase 1; SCP1; Small CTD phosphatase 1; CTDSP1; NIF3, NLIIF, SCP1

Immunogen: Synthetic peptide, corresponding Human CTDSP1.

Conjugate: Unconjugated

Modification: Unmodified