Product Description

cTnI (Phospho-S22/S23) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS64505-50, BS64505-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P19429

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB:1:500~1:1000 IHC:1:50~1:200

Background: Troponin I is part of a heteromeric complex playing an important role in the regulation of skeletal and cardiac muscle contraction. It consists of three subunits, troponin I (TnI), troponin T (TnT) and troponin C (TnC). Each subunit is responsible for part of troponin complex function. TnI inhibits ATPase activity of acto myosin and TnT and TnI are present in cardiac muscles in different forms than in skeletal muscles. Only one tissue specific isoform of TnI is described for cardiac muscle tissue (cTnI) and this is expressed only in myocardium.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: cTnI (Phospho-S22/S23) polyclonal antibody detects endogenous levels of cTnI protein only when phosphorylated at Ser22/Ser23.

Bioworld Molecular Weight: ~ 28 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of cTnI (Phospho-S22/S23) polyclonal antibody at 1:500 dilution
Lane A: The Heart tissue lysate of Mouse
Lane B: The Kidney tissue lysate of Mouse
Lane C: The Heart tissue lysate of Rat
Lane D: The Kidney tissue lysate of Rat

Alternative Name: Troponin I, cardiac muscle; Cardiac troponin I; TNNI3; TNNC1

Immunogen: Synthetic phosphopeptide derived from human cTnI around the phosphorylation site of Serine 22/Serine 23.

Conjugate: Unconjugated

Modification: Phosphorylation