Product Description

CUL1 (Y761) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1081-50, BS1081-100

Sizes: 50µl, 100µl

Swiss-Prot: Q13616

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: CUL-1, which is the mammalian homolog of yeast Cdc53, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1-mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3, facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Cullin 1 (Y761) polyclonal antibody detects endogenous levels of Cullin 1 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CUL1 (Y761) pAb at 1:500 dilution
Lane 1: The Brain tissue lysate of Rat (40ug)
Lane 2: The Lung tissue lysate of Rat (40ug)
Lane 3: The Lung tissue lysate of Mouse (40ug)
Lane 4: A549 whole cell lysate (40ug)
Lane 5: HCC827 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of CUL-1 (Y761) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 90 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: CUL 1; CUL-1; CUL1; CUL1; Cullin-1; Cullin1; MGC149834; MGC149835;

Immunogen: Synthetic peptide, corresponding to amino acids 728-780 of Human CUL-1.

Conjugate: Unconjugated

Modification: Unmodified