CUL2 (I726) Polyclonal Antibody-BS1082

CUL2 (I726) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1082-50, BS1082-100

Sizes: 50µl, 100µl

Swiss-Prot: Q13617

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: Cullin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)-mediated proteolysis. CUL-1, which is the mammalian homolog of Cdc53 from yeast, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCf (Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1 mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3 facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase. Proteolysis also occurs by way of CUL-4 associating with Nedd-8, a ubiquitin- like protein, where it too functions as an active component of a multifunctional E3 complex. CUL-5, or vasopressin-activated, calcium-mobilizing protein (VACM-1), is also included in the cullin family as it shares substantial sequence homology with CUL-1.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Cullin 2 (I726) polyclonal antibody detects endogenous levels of Cullin 2 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CUL 2 (I726) pAb at 1:500 dilution
Lane 1: SGC7901 whole cell lysate (40ug)
Lane 2: HEK293T whole cell lysate (40ug)
Lane 3: The Kidney tissue lysate of Rat (40ug)
Lane 4: The Kidney tissue lysate of Mouse (40ug)
Lane 5: MCF-7 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of CUL-2 (I726) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 82,84 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Cullin 2; Cullin2; CUL2; CUL 2; CULLIN-2; MGC131970

Immunogen: Synthetic peptide, corresponding to amino acids 696-742 of Human CUL-2.

Conjugate: Unconjugated

Modification: Unmodified